TFSQ型高效环保组合式粮食杂质清理筛的研制

通过对振动筛理、垂直风选、旋风分离与沉降系统的研究,研制出集振动筛理、风选、旋风分离沉降、含尘气流循环再利用为一体的TFSQ型高效环保组合式粮食杂质清理筛。经应用测试和专家鉴定:该设备具有清理效率高、清理过程粉尘污染小,使用维护方便、可靠性强等优点,具有广阔的市场推广前景。     

芒属植物叶绿体InDel标记的开发与应用

研究利用芒属植物(芒02381和荻04005)叶绿体全基因组测序的结果,开发12对InDel标记。采用正交设计法优化cpInDelPCR体系,得到模板DNA、dNTPs、Mg2+、引物、TaqDNA聚合酶5个关键因素在体系中的最佳组合。12对InDel标记对43份芒属和2份甘蔗材料进行扩增,共扩增出538条条带,其中3对引物扩增出13条多态性条带,占总数的2.4%。将所有位点读带转化为数值矩阵并用UPGMA法聚类,在遗传相似系数1.4的水平上,和南荻聚为一类,而芒和与五节芒、双药芒、红山茅、尼泊尔芒、甘蔗聚为一类,4个具有杂交种特征的材料单独聚为一类。试验开发的芒属植物cpInDel标记可用于芒与荻的区分,指导芒属植物的杂交育种。     

一种简易的花房温湿度无线自动监控系统

花房内的温湿度变化直接影响到花卉的健壮生长,以C8051F330为核心设计一款简易的花房温湿度无线自动监控系统,可对花房内的温湿度进行实时自动监控,当花房内温湿度指标超过设定值时,系统发出声光报警。该系统温度测量范围为-55~125℃,精度为±4℃;相对湿度测量范围为10%~90%,精度为±5%。经测试,系统运行稳定,...     

土壤外源钴对大麦根伸长的毒害及其预测模型

选取我国11种不同性质的农田土壤，通过外源添加重金属钴（Co），研究其对大麦（Hordeum vulgare L.）根伸长的毒性阈值及土壤性质对Co毒性的影响。结果发现，Co对大麦根伸长10%抑制效应（EC₁₀）在11种土壤中的变化范围为37.1~3 914 mg·kg^-1土（105.5倍），50%抑制效应（EC₅₀）的变化范围为166.1~6 030 mg·kg^-1土（36.3倍）。建立土壤性质与毒性阈值的回归方程，结果表明土壤pH是影响土壤Co毒性阈值最重要的因子，作为单因子时分别可以解释77.6%、72%的EC₁₀和EC₅₀的变异（P≤0.001）。当在EC₁₀预测模型中引入土壤pH和土壤黏粒（Clay）双因子时，可以解释83.9%的EC₁₀的变异（P<0.001），EC₅₀预测模型中引入土壤pH和总碳（TC）双因子时，可以解释86.1%的EC₅₀的变异（P<0.001）。将我国土壤中得到的Co毒性阈值预测模型和欧洲北美10种土壤的预测模型进行比较验证，结果发现基于我国土壤得到的预测模型可以较为准确地预测欧洲北美土壤中Co的大麦根伸长毒性阈值，但基于欧洲北美土壤的预测模型不能准确预测我国土壤中Co的毒性阈值。研究表明，我国土壤性质对Co毒性有显著的影响，基于土壤性质建立的预测模型可为土壤中Co生态风险评价提供参考依据。     

鸡粪除臭菌的分离筛选及除臭效果分析

以鸡粪堆肥为材料,筛选高效除臭菌株并构建复合菌系,为鸡粪的无害化处理提供具有除臭功能的菌种资源。首先,采用驯化富集法、平板稀释法、产气试验、除氨试验、除硫化氢试验以及纤维素降解试验筛选出鸡粪堆肥中的除臭菌;其次,通过16S rRNA基因测序鉴定菌株;最后,通过拮抗试验构建除臭菌系。研究分离得到5株具有除臭功能的菌株,分属于芽孢杆菌属(MS03,Bacillus sp.)、贝莱斯芽孢杆菌(MS07,Bacillus velezensis)、耐寒短杆菌(MS11,Brevibacterium frigoritolerans)、木糖葡萄球菌(MS42,Staphylococcus xylosus)和变异棒杆菌(MS82,Corynebacterium variabile)。复合菌系C2(MS03+MS07)和C4(MS03+MS82)的综合除臭率均高于60%,且明显高于单株菌株及其他复合菌系。研究表明,这两个由不同除臭菌组合而成的复合菌系在无害化处理鸡粪方面具有较大的潜力和应用前景。     

馥郁滇丁香新品种'香波'的选育

'香波'是从馥郁滇丁香(Luculia gratissima (Wall.) Sweet.)野生种实生群体中选择出的特异单株,经无性繁殖培育而成的无性系新品种.多年生常绿灌木,株高可达2～3 m;叶片纸质,椭圆形,长(16.68±1.35)cm,宽(7.76±0.78)cm,叶背面被秕糠状毛;盛花期花萼形态合抱;花裂片腹面浅紫粉色,花裂片卵圆形,边缘具波折;花冠裂片间无附属物,长花柱,观赏性强.     

射流泵装置性能预测方法

提出了一种射流泵装置性能预测方法,并进行了试验验证.以射流泵试验所得射流泵的流量比与压力比曲线,以及离心泵的流量扬程曲线作为预测初始条件,通过射流泵装置2种吸上高度4.5和9.0 m的性能试验,比较各流量比时装置工况点试验值与预测值精度,发现数值解法整体误差较小,能更好地反映射流泵扬程随流量比变化的情况,但与试验值相比仍存在误差且个别工况点误差较大,需进一步修正.引入预测值与试验值的比值作为修正系数,通过Plackett-Burman试验设计,从吸上高度、面积比、喷嘴直径、流量比、喉嘴距、喉管长径比、泵转速等因子中筛选出对射流泵扬程影响效应显著的面积比及流量比作为修正公式的关键参数,利用遗传算法和公式自动搜索拟合,得到射流泵扬程的计算公式,且相关系数超过0.99.通过射流泵装置在吸上高度为3.5和8.0 m的试验结果比较,表明具有较高的可信度.     

Effects of remifentanil on monophasic action potential and transmural dispersion of repolarization in rabbit myocardium

AIM: To study the effect of remifentanil on monophasic action potential and transmural dispersion of repolarization (TDR) in the 3-layer myocardium of isolated rabbit hearts. METHODS: Adult rabbits (n=18, 2.0 ~ 2.5 kg) were used to isolate the hearts for preparing Langendorff perfusion model. The hearts were randomly divided into 3 groups after perfusion with K-H solution for 15 min: the perfusion in control group (C group) continued for 60 min; the hearts in remifentanil group (R group) were perfused with 12 μg/L remifentanil K-H solution for 60 min; the hearts in remifentanil+aminophylline group (RA group) were given 60-min perfusion of 12 μg/L K-H remifentanil+30 mg/L aminophylline. The HR and 3 layers of myocardial monophasic action potential (MAP) in the left ventricular anterior wall were recorded at time points after balanced infusion for 15 min (T₀), and continued perfusion for 15 min (T₁), 30 min (T₂) and 60 min (T₃). The monophasic action potential duration of repolarization at 90% (MAPD₉₀) and the transmural dispersion of repolarization (TDR) were calculated. The early afterdepolarization, delay afterdepolarization and arrhythmia were also observed. RESULTS: In R group, slower HR and prolonger MAPD₉₀ and TDR at T₁~T₃ were observed as compared with those at T₀ (P<0.05). R group showed slower HR and longer MAPD₉₀ and TDR than C group and RA group (P<0.05). CONCLUSION: Remifentanil slows the HR, extends the MAPD₉₀ and increases the TDR, thus being prone to induce reentry. Aminophylline makes HR faster and MAPD₉₀ shorter, thereby reducing the TDR.     

菊花花瓣伸长相关基因CmXTHs的克隆与功能验证

从杭白菊(Chrysanthemum morifolium‘Hangbaiju’)中克隆了4个细胞壁松弛和伸展相关的木葡聚糖内转糖苷酶/水解酶基因,分别命名为CmXTH1、CmXTH2、CmXTH3和CmXTH4,序列分析表明其编码的氨基酸序列N端都含有23~30个氨基酸组成的信号肽区域和保守的催化活性位点DE(I/L)DEFLG以及紧邻的N(R/A)T组成的N端糖基化位点,CmXTH1/2/3的C端均含有4个保守的半胱氨酸残基,CmXTH1/2/3/4蛋白的相似度为66.2%。系统进化分析结果表明,CmXTH1/2/3属于XTH家族Ⅰ组,CmXTH4属于Ⅱ组。实时荧光定量结果表明:(1)菊花CmXTH1/2/3/4在根、茎、叶和花蕾中均有表达。CmXTH1在根中表达量较高,CmXTH2/3在茎中表达量较高,CmXTH4在花蕾中表达量最高。(2)CmXTH1/2/3/4在花序不同部位表达量有差异,CmXTH1/4在舌状花中表达量最高,CmXTH2/3在筒状花中表达量较高。(3)CmXTH1/2/3/4在舌状花不同发育阶段表达量差异显著,CmXTH1/2在盛花期表达量最高,CmXTH3在衰老期表达量最高,CmXTH4在初开期表达量最高。病毒诱导基因沉默结果表明,CmXTH1/2/3/4沉默系的花序直径和舌状花长度与对照相比均有所减小,其中CmXTH4沉默系减小最大,分别减小了25.67%和10.42%,舌状花花瓣表皮细胞与对照相比明显变小,影响了舌状花花瓣的伸长;CmXTH2沉默系的筒状花雄蕊长度和CmXTH4沉默系的花萼直径分别与对照相比显著减小。这表明菊花CmXTH1/2/3/4基因参与了花序的开放,促进了舌状花花瓣的伸长,对于菊花的花序增大具有重要作用。     

Expression,Purification and Activity Evaluation of Mb1230 Gene of Mycobacterium bovis

To explore the value of Mb1230 protein of Mycobacterium bovis in the diagnosis of bovine tuberculosis,we obtained the Mb1230 gene by PCR and constructed recombinant plasmid pET-22b-Mb1230.Recombinant Mb1230 protein was obtained by IPTG induction and purified by affinity chromatography.The activity of the recombinant protein was evaluated by TST test,IGRA test and indirect ELISA.The size of the recombinant protein matched with the theoretical value proved by SDS-PAGE;Western blotting result showed that the recombinant protein could react with mouse anti-His antibody,and had specific band;The results of TST test,IGRA test and indirect ELISA test also showed the recombinant protein had antigenic activity.The results indicated the recombinant protein Mb1230 had good B cell and T cell activity,so,it had the potential application in the diagnosis of bovine tuberculosis.